Biodegradation of Petroleum Sludge by Methylobacterium sp. Strain ZASH

Shamsul Harumain, Zakuan Azizi and Mohamad, Mohd Azrul Naim and Nordin, Noor Faizul Hadry and Abd Shukor, Mohd Yunus (2023) Biodegradation of Petroleum Sludge by Methylobacterium sp. Strain ZASH. Tropical Life Sciences Research, 34 (2). pp. 197-222. ISSN 1985-3718 E-ISSN 2180-4249

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Abstract

A bacterium was isolated from sludge-contaminated soil in a petroleum refinery and tested for its ability to degrade aliphatic hydrocarbon compounds present in petroleum sludge. The isolate was grown on minimal salt media agar supplemented with 1 % (w/v) petroleum sludge. The isolate was tentatively identified as Methylobacterium sp. strain ZASH based on the partial 16s rDNA molecular phylogeny. The bacterium grew optimally between the temperatures of 30 °C and 35 °C, pH 7 and 7.5, 0.5 and 1.5 % (v/v) Tween 80 as the surfactant, and between 1 and 2 % (w/v) peptone as the nitrogen source. The constants derived from the Haldane equation were ?max = 0.039 hr-1, Ks = 0.385 % (w/v) total petroleum hydrocarbons (TPH) or 3,850 mgl-1 TPH, and Ki =1.12 % (w/v) TPH or 11,200 mgl-1. The maximum biodegradation rate exhibited by this strain was 19 mg l-1 hr-1 at an initial TPH concentration of 10,000 mg l-1. Gas chromatography analysis revealed that after 15 days the strain was able to degrade all aliphatic n-alkanes investigated with different efficiencies. Shorter n-alkanes were generally degraded more rapidly than longer n-alkanes with 90% removal for C-12 compared to only 30% removal for C-36. The addition of sawdust did not improve bacterial degradation of petroleum hydrocarbons, but it assisted in the removal of remaining undegraded hydrocarbons through adsorption.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Petroleum Sludge Biodegradation Kinetics Sawdust Methylobacterium sp.
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Zakuan Azizi Shamsul Harumain
Date Deposited:	02 May 2023 16:52
Last Modified:	02 Jan 2024 09:38
URI:	http://irep.iium.edu.my/id/eprint/104387

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