Neuroprotective effect of phospholipase A2 from Malaysian Naja Sumatrana venom against H2O2-induced cell damage and apoptosis

Abdullah, Nur Atiqah Haizum and Sainik, Nur Qisya Afifah Veronica and Esa, Ezalia and Muhamad Hendri, Nur Afrina and Ahmad Rusmili, Muhamad Rusdi and Hodgson, Wayne C. and Shaikh, Mohd Farooq and Othman, Iekhsan (2022) Neuroprotective effect of phospholipase A2 from Malaysian Naja Sumatrana venom against H2O2-induced cell damage and apoptosis. Frontier of Pharmacology, 13. pp. 1-14. ISSN 1663-9812

Preview	PDF (SCOPUS) - Supplemental Material Download (230kB) | Preview
	PDF (Article) - Published Version Restricted to Repository staff only Download (3MB) | Request a copy

Abstract

Oxidative stress is one of the factors involved in the pathogenesis of several neurodegenerative diseases. It has been reported that a secretory phospholipase A2 known as A2-EPTX-NSm1a has lower cytotoxicity in neuronal cells compared to its crude Naja sumatrana venom. In this study, A2-EPTX-NSm1a was tested for its neuroprotective activity on human neuroblastoma cells (SH-SY5Y) differentiated into cholinergic neurons against oxidative stress induced by hydrogen peroxide (H2O2). H2O2 treatment alone increased the caspase-3 and caspase-8 activities, whereas pre-treatment with A2-EPTX-NSm1a reduced the activity of these apoptosis-associated proteins. Moreover, A2-EPTX-NSm1a protects the morphology and ultrastructure of differentiated SH-SY5Y cells in the presence of H2O2. Oxidative stress increased the number of small mitochondria. Further evaluation showed the size of mitochondria with a length below 0.25 µm in oxidative stress conditions is higher than the control group, suggesting mitochondria fragmentation. Pre-treatment with A2-EPTX-NSm1a attenuated the number of mitochondria in cells with H2O2 Furthermore, A2-EPTX-NSm1a altered the expression of several neuroprotein biomarkers of GDNF, IL-8, MCP-1, TIMP-1, and TNF-R1 in cells under oxidative stress induced by H2O2. These findings indicate that anti-apoptosis with mitochondria-related protection, anti-inflammatory effect, and promote expression of important markers for cell survival may underlie the neuroprotective effect of A2-EPTX-NSm1a in cholinergic rich human cells under oxidative stress, a vital role in the neuronal disorder.

Item Type:	Article (Journal)
Uncontrolled Keywords:	neuroprotection, snake venom phospholipase A2, neurodegenerative disease, apoptosis, mitochondria, inflammation
Subjects:	R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences Kulliyyah of Pharmacy
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	14 Feb 2023 16:11
Last Modified:	06 Jun 2023 12:44
URI:	http://irep.iium.edu.my/id/eprint/103691

Actions (login required)

View Item