On-site sequencing of Polyporales sp. isolated from a rare lung fungal infection using Oxford nanopore flongle

Zainulabid, Ummu Afeera and Abdul Azih, Muhammad Naimmuddin and Abd. Fuaat, Azliana and Abidin, Mohd Radhwan and Ahmad, Hajar Fauzan (2023) On-site sequencing of Polyporales sp. isolated from a rare lung fungal infection using Oxford nanopore flongle. In: 3rd Symposium on Industrial Science and Technology (SISTEC2021), 25th - 26th August 2021, Virtual Conference.

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Abstract

Fungal infection is rarely reported in immunocompetent retroviral disease patients. We report a first case of a 42- year-old man with underlying retroviral disease on Highly Active Antiretroviral Therapy (HAART) who was infected with pulmonary Polyporales sp. Despite undetected viral load with high CD4 count, the patient was found to have unexplained significant loss of weight and appetite. Subsequently, CT thorax revealed multiple lung nodules at the lateral segment of the right middle lobe of lung. Bronchoscopy was carried out and showed a black patch at the right bronchus intermedius, lateral segment of the middle lobe which did not disappear upon bronchial flush or wash. The fungal culture was first described as Cladosporium sp. based on colony appearance and morphology under light microscopy examination. Due to the rarity of this infection, we recovered the sample from paraffin embedded tissue block and then recultured on Sabouraud agar at 300 Celcius. The total genomic DNA was then extracted by using the DNeasy Blood and Tissue kit. Full-length genome sequencing was carried out using Flongle platform for long read protocols which resulted as Polyporales sp. This finding will definitely add a novel value for precision diagnosis and clinical surveillance of a rare fungal infection.

Item Type:	Conference or Workshop Item (Other)
Uncontrolled Keywords:	Polyporales sp; Flongle
Subjects:	R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC111 Infectious and Parasitic Diseases
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Internal Medicine
Depositing User:	Asst. Dr. Ummu Afeera Zainulabid
Date Deposited:	09 Feb 2023 14:43
Last Modified:	30 May 2023 15:19
URI:	http://irep.iium.edu.my/id/eprint/103645

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