De novo transcriptome dataset of gene expression in Durio zibethinus during the infection of Phytophthora palmivora

Misman, Nadhirah and Samsulrizal, Nurul Hidayah and Noh, Abdul Latif and Wahab, Mohd Aswad and Ahmad, Khairulmazmi and Ahmad Azmi, Nur Sabrina (2022) De novo transcriptome dataset of gene expression in Durio zibethinus during the infection of Phytophthora palmivora. In: The 4th International Conference on Biosciences & Medical Engineering (ICBME 2022), 21-22 June 2022, Virtual. (Unpublished)

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Abstract

Phytophthora palmivora is a notorious oomycete that causes fatal diseases in tropical perennial crops. Over time, extensive literatures have been published on the destructive diseases caused by P. palmivora in Durio zibethinus (durian). The sequencing and analyzing of virulence-related genes that are involved during the infection of P. palmivora in durian will give a comprehensive understanding about the gene expression and infection mechanism. Healthy durian leave samples were inoculated with P. palmivora zoospores and harvested at 3 days-post-inoculation. The process involved transcriptome sequencing using Illumina RNA-seq technology and de novo assembly of the transcripts. The transcriptomic analysis managed to annotate 75,945 unigenes and 95,158 unigenes for control and infected sample respectively. The total length, N50 and GC content of Unigenes for control sample are 52,649,717, 1087 and 41.91% while for infected samples are 58,582,609, 911 and 43.24% respectively. 9 necrosis-inducing-Phytophthora-protein 1 (NPP1) genes, 17 elicitins and 45 RxLR proteins are the virulence-related genes acquired through data mining. This transcriptome data will be used to create novel control strategies for P. palmivora diseases in durian using molecular technology, resulting in increased durian production in durian-producing countries. The potential new control measures can also be a great benefit for farmers to minimize their exposure to hazardous fungicides.

Item Type:	Conference or Workshop Item (Slide Presentation)
Uncontrolled Keywords:	De novo assembly, RNA-Seq, Phytophthora palmivora, durian, gene discovery
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Nur Sabrina Ahmad Azmi
Date Deposited:	16 Jan 2023 15:02
Last Modified:	16 Jan 2023 15:02
URI:	http://irep.iium.edu.my/id/eprint/102656

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