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Isocratic RP-HPLC Method for the Simultaneous Determination of Reaction Rate in N-phenylbenzamide Synthesis

Helal Uddin, A.B.M. (2021) Isocratic RP-HPLC Method for the Simultaneous Determination of Reaction Rate in N-phenylbenzamide Synthesis. In: The 7th International Conference on Advancement in Science and Technology (iCAST2021), Kuantan, Pahang, Malaysia.

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Antiviral activity against Hepatitis C virus (HCV) and Enterovirus 71 (EV 71) has been discovered in a sequence of novel N-phenyl benzamide (Benzanilide) derivatives. Recently, such findings inspired researchers to look for more potent antiviral agents through the N-phenyl benzamide synthesis. A high-pressure liquid chromatography method has been developed to observe the rate of N-phenyl benzamide synthesis. This is the first time reporting the approach has been established together for N-phenyl benzamide and aniline or phenylamine in an amide coupling reaction. These compounds were detected on a reversed-phase column (C18) using a 50:50 mobile phase of acetonitrile - 10mM (PH 5) sodium acetate buffer and UV detection is recorded at 254 nm. At a temperature of 30°C, isocratic elution was employed with a flow rate of 0.7 ml/min and an injection volume of 20 µL. The retention time for aniline and N phenyl benzamide was ±4.10 and ±7.60 min respectively. A linear relationship (r2=0.9998 for benzanilide and r2=0.9992 for aniline) over the concentration range 10-100 μg/ml was found when the peak area was plotted against concentration. The percentage of RSD was also 0.23 and 0.85 for benzanilide and aniline respectively. The method was verified in accordance with the International Conference on Harmonization (ICH) guidelines. The precise, linear, and more accurate method was applied to find out the reaction rate of aniline to produce the N-phenyl benzamide.

Item Type: Conference or Workshop Item (Invited Papers)
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 03 Jan 2023 15:20
Last Modified: 03 Jan 2023 15:20
URI: http://irep.iium.edu.my/id/eprint/102335

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