Antimicrobial activity of β-Sitosterol Isolated from Kalanchoe tomentosa leaves against Staphylococcus aureus and Klebsiella pneumonia

Anwar, Risyandi and Sukmasari, Susi and Siti Aisyah, Lilis and Puspita Lestari, Faridia and Ilfani, Delia and Febriani Yunand, Yenny and Diki Presyta, Pebrian (2022) Antimicrobial activity of β-Sitosterol Isolated from Kalanchoe tomentosa leaves against Staphylococcus aureus and Klebsiella pneumonia. Pakistan Journal of Biological Science, 25 (7). pp. 602-607. ISSN 1028-8880 E-ISSN 1812-5735

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Abstract

Background and Objective: Fresh K. tomentosa leaves obtained from Bandung, Indonesia was extracted using n-hexane followed by serial dichloromethane maceration. Material and Method: N-hexane and ethyl acetate were used to separate the dichloromethane extract using Vacuum Liquid Chromatography and the isolated compounds were recrystallized with n-hexane. Result: 37 mg of dichloromethane extract was obtained from the extraction process. Recrystallized compound isolates were identified as stigmast-5-en-3ꞵ-ol or β-sitosterol. Both dichloromethane extract and β-sitosterol isolated compounds showed strong bacteriostatic activity against S. aureus with MIC=15.63 µg/mL and 7.81µg/mL, and K. pneumonia with MIC=7.81µg/mL and 31.25µg/mL, respectively. However, only dichloromethane extract exhibited a bactericidal effect (7.81 µg/mL). Conclusion: The pure β-sitosterol compound was isolated from K. tomentosa dichloromethane extract. Both the dichloromethane extract and the isolated β-sitosterol compound had antibacterial effects against S. aureus and K. pneumonia

Item Type:	Article (Journal)
Uncontrolled Keywords:	Kalanchoe tomentosa, β-sitosterol, Oral infection, Minimum Inhibition Concentration, Minimum Bactericidal Concentration
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Paediatric Dentistry and Dental Public Health
Depositing User:	Dr. Susi Sukmasari
Date Deposited:	07 Nov 2022 09:18
Last Modified:	07 Nov 2022 09:19
URI:	http://irep.iium.edu.my/id/eprint/100938

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