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In vitro callus induction of Plukenetia Volubilis (Sacha Inchi), a PUFA-rich plant

Mohd Jalil, Jasrina and Abdul Wahab, Widya and Meenakshi Sundram, Tamil Chelvan (2021) In vitro callus induction of Plukenetia Volubilis (Sacha Inchi), a PUFA-rich plant. In: International Conference on Bioscience and Biorefinery 2021, 13-14 October 2021, Bandung, Indonesia. (Unpublished)

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The young leaves and ovules of Plukenetia volubilis were cultured in MS medium under 5 different treatments of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) incubated in different conditions, either 24 hours light or 24 hours dark condition to develop an in vitro micropropagation through callus induction. Despite a series of contamination after surface sterilization, the young leaves explants deemed promising in developing callus in media supplemented with both plant growth regulators of 0.1 mg L-1 2,4-D + 0.05 mg L-1 BAP and 1.0 mg L-1 2,4-D + 0.05 mg L-1 BAP. Interestingly, it is the similar concentration that yielded callus from ovule. All treatments developed callus from ovule, in which the best callus was induced from MS media supplemented with 0.1 mg L-1 2,4- D + 0.05 mg L-1 BAP in both light and dark conditions compared to the single use of 2,4-D. This is the first report attempted on using ovules of sacha inchi as explant.

Item Type: Conference or Workshop Item (Other)
Uncontrolled Keywords: Sacha Inchi, In vitro culture, PUFA-rich plant, callus, ovul
Subjects: Q Science > QH Natural history > QH301 Biology
S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Plant Science
Depositing User: Dr Tamil Chelvan Meenakshi Sundram
Date Deposited: 13 Oct 2022 15:20
Last Modified: 13 Oct 2022 15:20
URI: http://irep.iium.edu.my/id/eprint/100541

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