Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species

Mohd Badri, Puteri Elysa Alia and Rismayuddin, Nurul Alia Risma and Mohd Kenali, Norzaiti and Darnis, Deny Susanti and Arzmi, Mohd Hafiz (2022) Characterization of Cervus timorensis velvet antler and its effect on biofilm formation of Candida species. Medical Mycology, 60 (9). pp. 1-13. ISSN 1369-3786 E-ISSN 1460-2709

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Abstract

Oral biofilms comprise extracellular polysaccharides and polymicrobial microorganisms. The objectives of the study were to characterize the deer velvet antler (DVA) compounds and their effect on Candida species biofilm formation with the hypothesis that DVA inhibits the biofilm of Candida spp. Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS) was conducted to characterize the DVA compounds. To study the effect of DVA on biofilm, Candida albicans ATCC MYA-4901 (ALT5), AIDS isolate (ALC2), oral cancer isolate (ALC3), C. dubliniensis ATCCMYA-2975,C.glabrataATCC90030,C.krusei14 243,C.lusitaniaeATCC34449,C.parapsilosisATCC22019,andC.tropicalisATCC13803 were inoculated with DVA in separate wells of a 96-well plate containing RPMI-1640 followed by 72 h incubation. A total of 45 compounds were detected in the DVA extract. C. lusitaniae exhibited a higher percentage of biofilm biomass reduction when treated with DVA extract (66.10% ± 5.33), followed by ALC3 (44.12% ± 6.24). However, C. glabrata, C. krusei, and C. parapsilosis showed no reduction in biofilm biomass after being treated with DVA extract. Most Candida strains also exhibited decreased total cell count when treated with DVA extract, except for ALC3 and C. krusei. ALT5 had the lowest total cell count (0.17 × 105 cells/ml) when cultured with DVA extract. In conclusion, DVA extract inhibits Candida spp. biofilm formation except for C. glabrata, C. krusei, and C. parapsilosis.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Deer velvet antler (DVA), Cervus timorensis, Candida species, biofilm
Subjects:	Q Science > QR Microbiology R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	AP Ts Dr Mohd Hafiz Arzmi
Date Deposited:	12 Oct 2022 09:21
Last Modified:	12 Oct 2022 09:22
URI:	http://irep.iium.edu.my/id/eprint/100533

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