Bahez, Ayesha and Wahid, Hanan Hamimi and Anahar, Fatin Najihah and Mustapha Rounal, Puteri Farah Diba and Mustafa Mahmoud, Mohammed Imad Al-Deen and Ismail, Hamizah (2022) The expression of virulence genes in Group B Streptococcus isolated from symptomatic pregnant women with term and preterm delivery. In: Malaysian Society for Microbiology Postgraduate Symposium (MSMPS2022), VIRTUAL. (Unpublished)
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Abstract
During pregnancy, group B streptococcus (GBS) colonization is one of the risk factors for preterm delivery and neonatal infections. Previous studies have revealed the crucial roles of GBS virulence factors including hemolytic pigment (CylE), hyaluronidase (HylB), serine rich protein (Srr) and bacterial surface adhesion of GBS (BsaB) in mediating GBS colonization and intrauterine ascending infection, that triggers preterm delivery. The aim of this study is to investigate the association between mRNA expression of virulence genes in GBS isolates obtained from symptomatic pregnant women and preterm delivery. GBS isolates were obtained from high vaginal swabs of pregnant women (n=40) with gestational age less than 37 weeks and symptoms including preterm labour, preterm premature rupture of membrane (pPROM), vaginal discharge and vaginal bleeding. RNA was extracted from these GBS isolates and RT-qPCR was performed to determine the relative mRNA expression of GBS virulence genes including CylE, HylB, Srr and BsaB. Women with preterm labour and pPROM who delivered prematurely were demonstrated with higher expression of CylE gene and a trend towards an increased expression of HylB gene, in comparison to women with term delivery. The expression of Srr and BsaB genes were both similar between symptomatic pregnant women who delivered at term and prematurely. These results suggest that following vaginal colonization, both CylE and HylB genes possibly contribute to intrauterine ascending infection and inflammation, causing preterm delivery in humans. These virulence factors may be targeted for the pre-clinical stages of vaccine development or therapeutic intervention.
| Item Type: | Conference or Workshop Item (Slide Presentation) |
|---|---|
| Subjects: | Q Science > QR Microbiology R Medicine > RG Gynecology and obstetrics |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | UNSPECIFIED |
| Depositing User: | Dr Hanan Hamimi Binti Wahid |
| Date Deposited: | 09 Dec 2022 08:10 |
| Last Modified: | 09 Dec 2022 08:10 |
| URI: | http://irep.iium.edu.my/id/eprint/100322 |
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