Proliferation of rat amniotic stem cell (AFSC) on modified surface microcarrier

Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Mohd. Salleh, Hamzah and Ariffin, Azmir (2021) Proliferation of rat amniotic stem cell (AFSC) on modified surface microcarrier. In: Multifaceted Protocols in Biotechnology, Volume 2. Springer Nature Switzerland, Cham, Switzerland, pp. 63-74. ISBN 978-3-030-75578-2

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Abstract

Traditionally, stem cells are grown in two-dimensional vessels, although this poses a major challenge in the clinical-grade production of a large number of stem cells and their derivatives to fulfill the dose requirements in clinical trials (at a scale of ~109–1010 cells). The cultivation of microcarriers in stirred bioreactors has been recognized as among the most promising strategies for the rapid scale-up of stem cell-derived therapeutics under controlled conditions. In addition to largescale production, microcarriers have been applied in cell delivery systems for in vivo transplantation, to enhance cell survival and engraftment. ‘Microcarrier’ is a term used to refer to microspheres that support cells in mammalian cell culture, in which cells grow as monolayers on the surface of the particles. Microcarriers are spherical particles with a size ranging between 100 and 200 μm. Due to their small size, microcarriers have a wide variety of applications, one of which is cell culture and tissue engineering. This study proposes a novel model system for the in vitro study of cell proliferation ability on microcarriers.

Item Type:	Book Chapter
Uncontrolled Keywords:	Microcarrier, Stem cell, Pluripotent, Embryonic body
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Dr Nurhusna Samsudin
Date Deposited:	18 Aug 2021 22:36
Last Modified:	23 Aug 2021 09:17
URI:	http://irep.iium.edu.my/id/eprint/91613

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