Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells

Adnan, Adek Zamrud and Bakhtiar, Muhammad Taher and Afriani, Tika and Roesma, Dewi Imelda and Putra, Andani Eka (2016) Cytotoxic activity assay of tinocrisposide from tinospora crispa on human cancer cells. Der Pharmacia Lettre, 8 (18). pp. 102-106. ISSN 0975-5071

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Abstract

Tinospora crispa is a climber plant belonging to the family of Menispermaceae. This plant has been used traditionally in Indonesia to treat some diseases and conditions such as fever, malaria, rheumatism, diabetes, cholera and inflammation. The aims of this study was to investigate cytotoxic activity of tinocrisposide extracted from the plant against human cancer cell lines. Cytotoxic activity of tinocrisposide in interval dose of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been investigated on H1299 and MCF-7 cell lines and their effects were quantified spectrophotometrically with the 3-(4,5 dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay at wavelength, λ 570 nm. Tinocrisposide in concentrations of 100; 50; 25; 12.5; 6.25; 3.125 μg/mL has been treated against H1299 cell line and showed a percentage viability of 55.5; 67.70; 80.84; 81.09; 82.69; and 87.06% respectively, with IC50 70.9 μg/mL, whereas on MCF-7 cell line showed percentage viability of 80.71; 89.30; 92.76; 96.01; 96.58, and 102.66% respectively, with IC50 > 100 μg/mL. It can be concluded that tinocrisposide has not shown any cytotoxic activity against H1299 and MCF-7 cell line, but it can be used as a chemopreventive agent toward those cell lines.

Item Type:	Article (Journal)
Additional Information:	5209/56396
Uncontrolled Keywords:	Cytotoxic activity, MTT assay,Tinocrisposide,Tinospora crispa
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy
Depositing User:	Dr Muhammad Taher
Date Deposited:	10 Apr 2017 14:33
Last Modified:	10 Apr 2017 14:33
URI:	http://irep.iium.edu.my/id/eprint/56396

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