Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables

Haron, Normah and Arcot, Jayashree and Bucknall, Martin (2015) Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables. In: Asian Congress on Biotechnology 2015, 15th-19th November 2015, Kuala Lumpur. (Unpublished)

PDF - Published Version Restricted to Repository staff only Download (4MB) | Request a copy

Abstract

Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds (9 phenolic acids and 5 flavonoids) in 85 minutes and uses deuterium-labelled 4-hydroxy-3-methoxy-d3-cinnamic acid (ferulic acid-d3) and 13C15-quercetin as internal standards. The results demonstrated that the method developed was reliable, specific and robust. Thus the method was successfully applied to analyse phenolic compounds in 18 different types of traditional Malaysian vegetables (both raw and processed) with 3 different fractions of phenolic acids. Gallic, ferulic, vanillic and caffeic acids were the most dominant phenolic acids, while quercetin, apigenin, kaempferol and luteolin were the predominant flavonoids in the samples studied.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7032/45944
Uncontrolled Keywords:	LC-MS/MS method,polyphenols, Malaysia, traditional vegetables
Subjects:	Q Science > Q Science (General) S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Normah Haron
Date Deposited:	24 May 2016 13:42
Last Modified:	05 Dec 2017 16:03
URI:	http://irep.iium.edu.my/id/eprint/45944

Actions (login required)

View Item